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Alexander V. Konarev

Leading Research Scientist
All-Russian Institute of Plant Protection (VIZR)
Podbelskogo 3, Pushkin,
St. Petersburg,
196608,
Russia

WELCOME
to Alexander Konarev's site. If you are interested in plant biochemistry, in particular in inhibitors of hydrolytic enzymes, please visit RESEARCH section. You can also look at CV and list of PUBLICATIONS of author. If you like adventures and prefer to travel by bike, canoe or foot in exotic places please visit Photo Gallery in JOURNEYS page.

RESEARCHES

I study plant proteins which inhibit hydrolytic  enzymes of various organisms:

Inhibitors are of interest as:

The inhibitors are well studied in various plants, but, generally, molecular structures of single inhibitors are studied much more then their natural variability in plant taxa.

The simple and sensitive methods for detection of  inhibitors of alpha-amylases, serine and cysteine proteinases and lectins among plant proteins separated by electrophoresis,  isoelectric focusing or thin layer gel-filtration were elaborated (Konarev, 1985, 1986; Konarev et al., 1991, 2000, 2002, Konarev and Lovegrove, 2012). These methods were used in study of the polymorphism and natural variability of main inhibitor systems in wild and cultivated species of wheat and related cereals, rice, potato,  Vigna and Phaseolus, sunflower, various Compositae, Apiaceae, other Asteridae and for identification of novel inhibitor types e.g. small (1.5 kDa) cyclic trypsin inhibitor from sunflower seeds, low-molecular trypsin Inhibitor with helix-turn-helix protease Inhibitory motif from seeds of Veronica (VhTI) or serine proteinase inhibitor from seeds of ancient gymnosperm plant, Cycas siamensis.
Detection methods were developed for proteinases as well that made it easier the identification and purification of a novel glutenin-specific salivary gland proteinase of Sunn pest (Eurygaster integriceps Put.) responsible for wheat gluten degradation and bread quality deterioration (Konarev et al., 2011, Konarev and Lovegrove, 2012).

 

EXAMPLES OF RESEARCHES


Polymorphism of insect alpha-amylase inhibitors in diploid wheats collected in Armenia and Azerbaidjan.
A, isoelectric focusing.
B, thin layer gel filtration. Inhibitors of Tenebrio molitor amylase were detected with use of PAAG/starch/amylase replicas



Variability of trypsin inhibitors in Vigna and Phaseolus species.
Isoelectric focusing of seed proteins followed by detection of inhibitors with use of gelatin replicas method.



The polymorphism of chymotrypsin/subtilisin inhibitors in several bread wheat varieties. Endosperm proteins were extracted with water and separated by isoelectric focusing in PAG. Protein bands were transferred from gel to the gelatine replica by diffusion and the latter was developed by chymotrypsin.

More than 500 species of Compositae and other Asteridae were screened for proteinase inhibitors. Cyclic low-molecular trypsin inhibitor consisting only of 14 amino acid residues was found only in seeds of Helianthus and Tithonia species. It appeared to be the smallest and most potent trypsin inhibitor ever found in plants.


Presentation of methods for detection of insect alpha-amylase and proteinase inhibitors among Vigna proteins in Laboratory of Plant Evolutionary Dynamics in NIAR, Head Dr. Duncan.A.Vaughan (right), Tsukuba, Japan.


KEY WORDS

insect amylase inhibitors, proteinase inhibitors, trypsin inhibitors,  chymotrypsin inhibitors,  subtilisin inhibitors, elastase inhibitors, cysteine proteinase inhibitors, papain inhibitors,  ficin inhibitors, lectins,  methods for detection of inhibitors,   plant  diversity, plant evolution,  plant systematics, plant resistance, cereals, legumes, potato, sunflower, Compositae, Asteridae, gymnosperms, Triticum, Aegilops, Secale, Hordeum, VignaPhaseolusOryzae, Helianthus, Carthamus, Cosmos, Zinnia, Centaurea, Cycas, Veronica, Anthriscus, Sunn pest Eurygaster integriceps glutenin-specific proteinase,


 

CURRICULUM VITAE

SPECIALIZATION

MAILING ADDRESS

Dr. Al.Konarev,
All-Russian Institute of Plant Protection (VIZR)
shosse Podbelskogo, 3, St.Petersburg 196608, RUSSIA
e-mail:   al_konarev@hotmail.com
home-page:   http://alvaskon.chat.ru

PERSONAL

Nationality:  Russian

EDUCATION

19.11.1992
Doctor of Sciences degree in biology, Scientific Council at the  Russian Academy of Sciences
A.N.Bach  Institute of Biochemistry (Moscow).
Thesis:  "Systems of hydrolase inhibitors in cereals: organisation, functions and evolutionary variability."

17.03.1983
PhD degree in biology, Scientific Council at the Leningrad (St. Petersburg) State University.
Thesis: "The nature of alpha-amylase inhibitors in relation to evolution and immunity of wheat and other cereals"

1979-1982
Postgraduate Courses at the All-Russian Institute for Plant Protection and at N.I.Vavilov Institute of Plant Industry,
Leningrad, USSR. Majoring in plant protein biochemistry, enzymology, systematics, genome analysis, plant immunity

1973-1978
MSc  degree in plant physiology and biochemistry, Leningrad State University, Faculty of Biology.
Thesis: "Insect and human  alpha-amylase inhibitors in wheat."

PRESENT POSITION

Leading Research Scientist, All-Russian Institute of Plant Protection,
Department of Entomology and Plant Immunity to Pests,
shosse Podbelskogo, 3, Pushkin, St.Petersburg 196609, RUSSIA
FAX: 7-812-470-51-10
Experimental base: N.I.Vavilov Institute of Plant Industry, Department of Molecular Biology, Bol. Morskaya str. 44, St.Petersburg 190000 RUSSIA

EMPLOYMENT RECORD

1982-2011
Research/Senior/Leading Research Scientist, All-Russian Institute for Plant Protection, Department of Entomology and Plant Immunity to Pests. Shosse; Podbelskogo, 3,Pushkin, St.Petersburg, 196609, RUSSIA

PRESENT AND CURRENT RESEARCH AREAS, SCHOLARLY ACTIVITIES,
PROJECTS AND GRANTS

2007-present time, research of plant proteinase inhibitors and insect digestive enzymes

2006-2007 Rothamsted Research, BBSRC Underwood fellowship (some results have been published in Phytochemistry, 2008 and J. Agric.Food.Chem., 2011)

2005 Rothamsted international fellowship (some results have been published in JBC, 2007)

2002
IACR-Long-Ashton Research Station, University of Bristol, UK
Royal Society grant for 6 months (Photo)
Study of proteinase inhibitors in various Asteridae in Crop Performance and Improvement Division (Head Prof. P.R.Shewry)
Results were published in Phytochemistry (2004)

2000
IACR-Long-Ashton Research Station, University of Bristol, UK
Royal Society grant for 6 months
Study of proteinase inhibitors in sunflower, safflower and other Compositae plants in
Seed Structure and Composition Lab (Head Prof. P.R.Shewry)
Results were published in Phytochemistry 59 (2002)

1997-1998
JIRCAS (Japan International Research Center of Agricultural
Sciences)  fellowship. National Institute of Agrobiological Resources, Plant Evolutionary Dynamics Lab., (Head Dr.Duncan Vaughan)
Tsukuba,  Japan
Responsible:  study  of proteinase and amylase inhibitors in  Vigna, Phaseolus and Oryzae species,  detection of inhibitors,  analysis of  their    variability and  interaction   with mammal,  microbial  and insect enzymes in relation with plant evolution and resistance to harmful organisms.  Results were reported on EUCARPIA Congress(Viterbo, Italy, 1998a) and published in EUPHYTICA, 2002

1995-1997
Grant from  Royal Society, Long-Ashton  Research  Station, (Photo)   Bristol,  UK (6 months total).   Participant of Joint Project  "SUNFLOWER PROTEINS".
Responsible:   study of proteinase inhibitors from sunflower: detection, purification, analysis of variability in cultivated and wild species,genetic analysis, interaction with proteinases from  pathogenic fungi. Discovering of low-molecular (1.5 kDa) TI in sunflower.
Study of genetic control and biochemical properties of sunflower albumins.
Results were reported at EUCARPIA Congress (Viterbo, Italy, 1998b) and published in
J Phytopathol (1999), J.Mol.Biol. (1999), Theor Appl Genet (2000) and Euphytica (2003)

1993-1994
Grant from Russian Fund for Fundamental Researches, All-Russian  Institute  for  Plant  Protection;  responsible:
analysis  of  heterogeneity,  variability  and  chromosome  control of insect and plant cysteine proteinase  inhibitors in wheat, rye, barley and other cereals.

1986-1992
Proteinase  inhibitors  in  vegetative  organs  of  cereals;  project leader; responsible: analysis of component  composition  and genetic control of trypsin  inhibitors  (TIs)  from  vegetative organs of wheat and related cereals.

1982-1992
TIs in endosperm  of  wheat  and  related  cereals;  project  leader; responsible: identification of TIs  among  endosperm proteins in di-, tetra- and hexaploid  wheats, purification  and studying of inhibitors  from  different wheat  species.
Analysis of inhibitors in species and varieties of Triticum  L., Aegilops L., Secale L. and other  cereals;  analysis  of  genome  and  chromosome  control  and  inheritance of  TIsstudy of defence role of TIs against insect pests.

1989-1991
Lectins  and  proteinase  inhibitors  in  cereals;   project  leader; responsible: development  of  simple  and  sensitive  methods of simultaneous detection of lectins and  proteinase  inhibitors in PAGE and  IEF  plant  protein patterns  (USSR patent). Research of lectins and comparison of  lectins  and  inhibitors in wheat and other cereals. Results were published in Biochemistry (Biokhimiya), Moscow, 1990)

1988-1990
Heterogeneity  of  hydrolases  in  relation  to  inhibitors:  project leader; responsible: elaboration of "cross"  methods of analysis of interaction of components of complex patterns  of enzymes with inhibitor components (USSR patent); studying of interaction of components of insect digestive and endogenous wheat alpha-amylases and proteinases with components of wheat inhibitors (Biochemistry (Biokhimiya), Moscow, 1991)

1984-1989
Inhibitors  of  chymotrypsin  and  subtilisin  in  cereals;  project  leader;  responsible:  analysis  of  heterogeneity, chromosome control, character of inheritance and evolutionary variability of inhibitors of chymotrypsin and  subtilisin in Triticum L., Aegilops L. and Secale L. species; purification and studying of biochemical properties  of  inhibitors;  elaboration of wheat variety identification method  by  IEF-patterns  of  chymotrypsin/subtilisin   inhibitors   (USSR  patent). Results were presented  at  the  III  International  Symp. Biochemical Identification of Varieties  (USSR, 1987)

1982-1990
Digestive proteinases of insects  and  their  inhibitors  in cereals; project leader; responsible: analysis of  digestive  proteinases of insects damaging wheat grain;  revealing  and  studying of wheat grain inhibitors  of  insect  proteinases; discovering the trypsin- and chymotrypsin-like and  cysteine digestive  proteinases in wheat pests. Results were  presented at the I International Symp. on Sunn pest (Turkey, 1988)

1982-1984
Inhibitors of insect and plant cysteine proteinases in wheat  and  related   cereals;   project   leader;  responsible:   discovering of inhibitors of cysteine proteinases of insects  and endogenous  cysteine  proteinase  of  germinating  wheat  grain  and  papain  in  wheats;  analysis  of  evolutionary  variability and chromosome control  of  cysteine  proteinase  inhibitors in wheat.

1982-1989
Methods for identification of proteinase inhibitors; project  leader; responsible:  elaboration  of  complex  of  high  sensitive, simple and universal methods  for identification  of  serine  and  cysteine  proteinase  inhibitors  after electrophoresis, isoelectric focusing  or  thin  layer  gel-filtration of plant or insect proteins (USSR patents) (Applied Biochemistry and Microbiology, 1985, and Biochemistry (Moscow), 1986)

1979-1982
Alpha-amylase inhibitors in wheats and other  cereals;  project  leader;  responsible:  elaboration  of simple and high  sensitive methods for detection of alpha-amylase  inhibitors  in protein patterns after electrophoresis, isoelectric focusing or thin layer gel-filtration (USSR  patents);  analysis of  insect  and mammal alpha-amylase inhibitors in wheat and  related cereals  (heterogeneity, evolutionary  variability,  chromosome control, inheritance); design of scheme of evolution of insect alpha-amylase inhibitor system in genus  Triticum L.;  discovering of endogenous wheat alpha-amylase inhibitors in wheats and other Triticeae Dum.  cereals (1981),  analysis of properties, distribution and variability of these inhibitors (1982).
 

INTERNATIONAL CONFERENCES

8th INTERNATIONAL WHEAT CONFERENCE, 14 June 2010 St. Petersburg, Russia
Alexander V. Konarev, Alison Lovegrove, Frederic Beaudoin, Justin Marsh, Nina A.
Vilkova, Ludmila I. Nefedova, Dilek Sivri Ozay, Hamit Koksel, Peter. R. Shewry Presentation: Characterization of a novel glutenin-specific proteinase of Sunn bug Eurygaster integriceps Put. responsible for wheat gluten degradation. (Abstract)

IS-MPMI 2009 XIV International Congress on Molecular Plant-Microbe Interactions
July 19-23, 2009, Quebec City, Canada
A.V. KONAREV, A.Lovegrove, P. R. Shewry
Presentation: Inhibitors of microbial serine proteinases in cycads and other gymnosperms (Abstract)

II VAVILOV INTERNATIONAL CONFERENCE 26-30 November 2007 GENETIC RESOURCES OF CULTIVATED PLANTS IN XXI CENTURY
Presentation PROTEINASE INHIBITORS IN STUDIES OF PLANT GENETIC DIVERSITY AND IMMUNITY

XI-th Int.CONGRESS ON PLANT-MICROBE INTERACTIONS (St.Petersburg, Russia, July 18-26, 2003).
Presentation: Distribution and polymorphism of fungal serine proteinase inhibitors in the Asteridae. (Abstract)

GLUTEN 2000, International Workshop (University of Bristol, April 2-7, 2000 ).
Presentation:  Insect and fungal enzyme inhibitors in study of variability, evolution and resistance of wheat and   other Triticeae Dum. cereals

THE MENDEL CENTENARY CONGRESS 2000 (100 YEARS OF GENETICS FOR PLANT BREEDING - MENDEL, MEIOSIS AND MARKER)
(Brno, Czech Republic, March 7-10, 2000).
Presentation: Insect and fungal enzyme inhibitors in study of plant variability and evolution.

XV EUCARPIA Congress ( Viterbo, Italy, 20-25 Sept.1998).
GENETICS  AND BREEDING FOR CROP  QUALITY AND RESISTANCE.
1. Variability of the inhibitors of serine, cysteine proteinases and insect  alpha- amylases in Vigna and Phaseolus
2. Polymorphism of  Inhibitors of  Hydrolytic Enzymes Present in Cereal and
Sunflower Seeds

XIV EUCARPIA Congress  ADAPTATION  IN  PLANT  BREEDING
(Juvaskyla, Finland, July 31-August 4., 1995).
Interaction of  insect  digestive  enzymes  with
protein inhibitors from plants and host-parasite coevolution

I INTERNATIONAL SYMPOSIUM ON SUNN  PEST  (Turkey,  Tekirdag, June 1988).  Inhibitors of Sunn pest (Eurygaster integriceps Put.) and other insect amylases and proteinases in wheat and related cereals


PUBLICATIONS

Papers

Al.V.Konarev and A.Lovegrove (2012) Novel Detection Methods Used in Conjunction with Affinity Chromatography for the Identification and Purification of Hydrolytic Enzymes or Enzyme Inhibitors from Insects and Plants. In: S. Magdeldin Ed. Affinity Chromatography, InTech, pp. 187-210 ISBN: 978-953-51-0325-7 (Full Text) (Get from InTech)

Al.V. Konarev, F. Beaudoin, J. Marsh, N.A. Vilkova, L.I. Nefedova, D. Sivri, H. Koksel, P.R. Shewry, and A.Lovegrove (2011) Characterization of a glutenin-specific serine proteinase of sunn bug Eurygaster integricepts Put. Journal of Agricultural and Food Chemistry, Vol: 59, Issue: 6, pp. 2462-2470 (http://pubs.acs.org/JAFC) (Full Text) (Supplementary Information)

N.A. Vilkova and A.V.Konarev (2010) Modern problems of plant immunity to pests. Plant Protection News (Vestnik Zaschity Rastenii), No. 3, pp. 3-15 (In Russian) (Full text)

Al.V.Konarev, A.Lovegrove, P.R.Shewry (2008) Serine proteinase inhibitors in seeds of Cycas siamensis and other gymnosperms. Phytochemistry, 69, pp.2482-2489

R.Conners, Al.V. Konarev, J.Forsyth, A.Lovegrove, J.Marsh, T.Joseph-Horne, P.Shewry, and R. L.Brady. (2007) An Unusual Helix-Turn-Helix Protease Inhibitory Motif in a Novel Trypsin Inhibitor from Seeds of Veronica (Veronica hederifolia L.) The Journal of Biological Chemistry vol. 282, no. 38, pp. 27760-27768 (Full Text_1) (Full Text_2)

Al.V. Konarev, J.Griffin, G.Yu. Konechnaya, P.R. Shewry (2004)The distribution of serine proteinase inhibitors in seeds of the Asteridae. Phytochemistry 65 (22):3003-20 (Abstract) (Full Text)

I.N.Anisimova, Al.V. Konarev, V.A. Gavrilova, V.T. Rozhkova, R.F. Fido, A.S. Tatham, P.R. Shewry (2003). Polymorphism and inheritance of methionine-rich 2S albumins in sunflower. Euphytica, 129 (1): 99-107 (Abstract)

Al.V.Konarev, I.N. Anisimova, V.A. Gavrilova, T.E. Vachrusheva, G.Yu. Konechnaya, M. Lewis, P.R. Shewry  (2002) Serine proteinase inhibitors in Compositae: distribution, polymorphism and properties. Phytochemistry, 59  279-291 (Full Text)

Al. V. Konarev, Tomooka N. & Vaughan D. A. (2002) Proteinase inhibitor polymorphism in the genus Vigna Savi subgenus Ceratotropis and its biosystematic implications. Euphytica, 123 (2): 165-177  (Full Text)

Al.V. Konarev (2002) Enzyme inhibitors as genetic markers. J. Agrarnaya Rossia, No 3, 44-52 (In Russian)

Al.V.Konarev, I.N.Anisimova, T.E.Vachrusheva, G.Yu. Konechnaya and  P.R.Shewry (2001) Inhibitors of fungal proteinases in safflower (Carthamus L.) and other Compositae. In: J.W.Bergman and H.H.Mundel ed. Proc. of Vth International Safflower Conference, Williston, North Dakota and Sidney, Montana USA, July 22-27, P.39-45

KonarevAl.V., D.A. Vaughan and P.R. Shewry (2000) Insect and fungal enzyme inhibitors in study of plant variability and evolution. Czech J. Genet. Plant Breed. 2000, vol. 36, p. 121-123. (Full Text)

Konarev Al. V. (2000) Insect and fungal enzyme inhibitors in study of; variability, evolution and resistance of wheat and other ;Triticeae Dum. Cereals. In: P.R.Shewry and A.Tatham ed. Wheat Gluten. Royal Society of Chemistry, UK, p. 526-530.

Konarev Al.V., Anisimova I.N., Gavrilova V.A., Vachrusheva T.E., Shashilova L.I., Konechnaya G.J., P.R. Shewry (2000) Heterogeneity and natural variability of proteinase inhibitors in sunflower (Helianthus L.) and other Compositae. Proc. of 15th Int. Sunflower Conference, (12-15th June, 2000, Toulouse, France), Vol.1., Section A, p. 109-114.

Anisimova I., Al. Konarev, S. Turkav, V. Gavrilova and P. Shewry (2000) Genetic studies on sunflower seed storage proteins. Proc. of 15th Int. Sunflower Conference, (12-15th June, 2000 Toulouse, France).

Konarev, Al.V., Anisimova, I.N., Gavrilova, V.A., Rozhkova, V.T., Fido, R., Tatham, A.S.,
and Shewry, P.R. (2000) Novel Proteinase Inhibitors in Seeds of Sunflower (Helianthus annuus L.): Polymorphism, Inheritance and Properties. Theoretical and Applied Genetics, 100, 82-88. (Full Text)

Al.V.Konarev (2000) Proteinase inhibitors and resistance of potato to Colorado potato beetle. In K.G.Skryabin and K.V.Novozhilov ed.: Modern systems of plant protection and novel approaches to improving of potato resistance to Colorado potato beetle. Moscow, Nauka, p.35-40. (In Russian)

Vaughan, D.A., N. Tomooka, Q. Xu, A. Konarev, K. Doi, K. Kashiwaba and A. Kaga. (2000) The Vigna angularis complex in Japan. In: 7th MAFF International Workshop on Genetic Resources Part 1. Wild Legumes. NIAR, Tsukuba, Japan, p. 159-176

Luckett S., Garcia R.S., Barker J.J., Konarev Al.V., Shewry P.R., Clarke A.R., Brady R.L. (1999) High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds. J Mol Biol 1999 Jul 9;290(2):525-33 (Full Text)

Konarev Al.V., I.N. Anisimova, V.A. Gavrilova, P.R.Shewry (1999) Polymorphism of Inhibitors of Hydrolytic Enzymes Present in Cereal and Sunflower Seeds. In: G.T.S.Mugnozza, E.Porceddu & M.A.Pagnotta ed. "Genetics and breeding for crop quality and resistance". Kluwer Academic Publishers, NL, 135-144. (Full Text)

Konarev Al.V., N.Tomooka, M. Ishimoto, and Duncan A.Vaughan (1999) Variability of the inhibitors of serine, cysteine proteinases and insect alpha-amylases in Vigna and Phaseolus. In: G.T.S.Mugnozza, E.Porceddu & M.A.Pagnotta ed. "Genetics and breeding for crop quality and resistance". Kluwer Academic Publishers, NL, 173-181. (Full Text)

Anisimova I.N., Konarev Al.V., Rozhkova V.T., Gavrilova, V.A., Fido R.J., Tatham A.S., Shewry P.R. (1999) Variability of seed storage proteins within the sunflower gene pool. In: G.T.S.Mugnozza, E.Porceddu & M.A.Pagnotta ed. "Genetics and breeding for crop quality and resistance". Kluwer Academic Publishers, NL, 331-338.

Konarev, Al.V., Kochetkov, V.V., Bailey, J.A., and Shewry, P.R. (1999) The detection of inhibitors of the Sclerotinia sclerotiorum (Lib) de Bary extracellular proteinases in sunflower, J. Phytopathology 147 No 2, p 105-108 (Full Text)

Vaughan D., Tomooka N., Konarev Al. (1998) Sights into variation in the CC genome wild relatives of rice. In: Research Reports of National Institute of Agriobiological Resources, Tsukuba, Japan, p. 36-39 (in Japanese).

Tomooka N., Vaughan D.A., Konarev Al. and Tsukamoto S. (1998) Collection of the wild relatives of crops,1997.2. The azuki bean (Vigna angularis var. angularis) genepool and soybean (Glicine max) genepool in the Central Region of Honshu, Japan 15-24th October 15-24th. In: Annual report on exploration and introduction of plant genetic resources. January 1998, V.14, p.71-83 (in Japanese with English summary).

Konarev Al.V. (1997) Hydrolase inhibitors in plant defence mechanisms. In: Russ. Plant Protection Congress. St.Petersburg: VIZR, P.229-234. (In Russian)

Konarev Al.V. (1996) Interaction of insect digestive enzymes with protein inhibitors from plants and host-parasite coevolution. Euphytica, 92, 89-94.

Konarev Al.V. (1994) Variability of hydrolase inhibitors and the problems of evolution, immunity and breeding of cereals. In: Konarev V.G. ed. Molecular biological aspects of applied botany, genetics and plant breeding. (Supplement to "Theoretical basis of plant breeding", Vol. 1). Sankt-Petersburg: VIR. P. 83-94.

Al.V.Konarev (1993) Component composition, genome and chromosome control of trypsin inhibitors from bread wheat leaves, roots and germs. Selskochozajstvennaya biologia (Skh.Biol). N 1. P.43-52. (In Russian)

Al.V.Konarev (1992) Analysis of native trypsin inhibitors from endosperm of wheats with various genome composition. Skh. Biol. N 5. P. 10-16. (In Russian)

Al.V.Konarev, Yu.V.Fomicheva (1991). Cross analysis of the interaction of alpha-amylase and proteinase components of insects with protein inhibitors from wheat endosperm. Biokhimiya (Biochemistry, Moskow). V.56. P. 628-638. (Abstract) Full Text (in English) (in Russian)

Al.V.Konarev (1990) Cross analysis of interaction of endogenous alpha-amylase and proteinase components with protein inhibitors in wheat. Doklady VASKhNIL. N 1. P. 10-13.

Al.V. Konarev (1990) Detection of lectins in analytical isoelectrofocusing of wheat proteins. Biokhimiya (Biochemistry, Moskow). V. 55. P. 1975-1983.

Al.V. Konarev (1989) The evolutionary variability and genetic control of chymotrypsin-subtilisin inhibitors in wheat. Doklady VASKhNIL. N 5.P. 8-10.

Al.V.Konarev (1989) Methods of two-dimensional fractionation of native proteinase inhibitors from seeds and leafs of wheat. In Modern methods of researches in plant science. Leningrad, Len.obl.pravl.Vses.agroprom.NTO. (In Russian)

Al.V.Konarev (1988) Polymorphism of chymotrypsin-subtilisin inhibitors and possibilities of its use in wheat variety identification. In Biochemical identification of varieties. Materials III international symposium ISTA. Leningrad, USSR, 1987), Leningrad, VIR. P. 176-181.

Al.V.Konarev (1988) Alpha-amylase and proteinase inhibitors and problems of immunity of cereals to pests and diseases. Bulletin VPS MOBB, V.24. P. 41-52. (In Russian)

Al.V.Konarev (1987) Hydrolase inhibitors in the study of the gene pool of wheat and its relative cereals. In: Protein markers in varietal identification and registration of genetic resources of cultivated plants (Bulletin of applied botany, genetics and plant breeding, vol.114) Leningrad, VIR. P.34-47. (In Russian)

Al.V. Konarev (1987) Variability of trypsin-like proteinase inhibitors in wheat and related cereals. Skh. Biol. N 5. P.17-24. (In Russian)

Al.V. Konarev, O.P. Mitrophanova(1987) Analysis of inheritance of components of alpha-amylase and trypsin inhibitors in bread wheat. Cytologia and Genetica . V.21. N 1.P.15-18.

Al.V.Konarev (1987) Component composition of trypsin inhibitors from grain and leaves of wheat. Doklady VASKHNIL, N 12. P. 6-9.

Al.V.Konarev (1986) Methods of identification of alpha-amylase inhibitors in electrophoretic, isoelectric and thin layer chromatographic protein patterns. In Biochemical methods of researches in entomology. Leningrad, VIZR, P.46-50. (In Russian)

Al.V. Al.V.Konarev (1986) Analysis of proteinase inhibitors from wheat grain by gelatin replicas method. Biokhimiya. V.51. P. 195-201.

Al.V. Konarev (1986) Relationship and variability of proteinase and amylase inhibitors in wheat and related cereals. Sch.Biol. N 3. P. 46-52. (In Russian)

Al.V.Konarev (1985) Methods for analyzing the component composition of cereal alpha-amylase and proteinase inhibitors. Prikladnaya Biokhimiya i Microbiologia (Applied Biochemistry and Microbiology, Moscow). V. 21. P. 92-100.

Al.V. Konarev (1984) Identification of inhibitors of thiol proteinases of insects and grain in wheat and other cereals. Doklady VASKhNIL. N 10. P. 13-15.

Al.V. Konarev, N.A.Vilkova (1984) Identification, component composition and functions of proteinase inhibitors in wheat. Skh. Biol. N 9. P. 39-44. (In Russian)

Al.V.Konarev, N.A.Vilkova (1984) Approaches to studying of defence role of insect enzyme inhibitors in cereals. In: Resistance of agricultural plants to pests and problems of plant protection (Leningrad, USSR, VIZR) (In Russian)

Al.V.Konarev, N.A.Vilkova (1984) Inhibitors of enzymes and plant immunity. Zaschita rastenij, N 10. P.17-19. (In Russian)

Al.V.Konarev (1982) Component composition and genetic control of insect alpha-amylase inhibitors from wheat and aegilops grain. Doklady VASKhNIL. 1982. 6. P. 42-44.

Al.V.Konarev (1982) Identification of inhibitors of endogenous and exogenous alpha-amylases among wheat proteins. Bulletin VIR. V. 118.P. 11-12. (In Russian)

Al.V.Konarev (1981) Specificity of wheat grain albumins (alpha-amylase inhibitors) towards enzymes of Sunn pest (Eurygaster integriceps Put.). Bulletin VIR. V. 114. P.30-32. (In Russian)

Abstracts

Al.V. Konarev, Kochetkov V.V., Shewry P.R. (2003) Distribution and polymorphism of fungal serine proteinase inhibitors in the Asteridae. XI-th Int.Congress on Plant-Microbe Interactions, July 18-26, 2003, St.Petersburg, Russia (Abstract)

Al. Konarev, D. Vaughan and P. Shewry (2000) Insect and fungal enzyme inhibitors in study of plant variabilityand evolution. In: Vortage fur Pflanzenzuchtung, vol 47, Poster No 130.

Al.V. Konarev (2000) Insect and fungal enzyme inhibitors in study of variability,evolution and resistance of wheatand other Triticeae Dum. Cereals. In: Poster Abst. Gluten 2000 International Workshop, Bristol, UK

Al.V. Konarev, D.A.Vaughan and P.R.Shewry (1999)Inhibitors of amylases and proteinases of insects and microorganisms in study of plant diversityand evolution Proc. XVI International Botanical Congress, (1-7 August 1999, St.Louis, Missouri USA), 1999

Konarev Al. V. , Anisimova I. N. , Tomooka Norihiko, Vaughan Duncan A., Shewry P. R. (1999).POLYMORPHISM OF INHIBITORS OF INSECT AND FUNGAL AMYLASES AND PROTEINASES IN CEREALS, LEGUMES AND SUNFLOWER.Abst. 9th International Congress on Molecular Plant-Microbe Interactions. Amsterdam, July 25-30 1999

Luckett S., R. Santiago Garcia, J.J. Barker, A.V. Konarev, A.R. Clarke, P. Shewry and R.L. Brady (1999)High Resolution structure of a potent, cyclic trypsin inhibitor from sunflower seeds Proc. The XXIV Lorne Conference on Protein Structure and Function (7-11 February, 1999, Lorne, Australia) A-6.

Al.V .Konarev, I.N. Anisimova, V.A. Gavrilova, P.R.Shewry (1998) Polymorphism of Inhibitors of Hydrolytic Enzymes Present in Cereal and Sunflower Seeds. Abst. XV EUCARPIA Congress (20-25 Sept.1998, Viterbo, Italy) "Genetics and breeding for crop quality and resistance". P.34.

Konarev Al.V., N.Tomooka, M. Ishimoto, and Duncan A.Vaughan (1998) Variability of the inhibitors of serine, cysteine proteinases and insect alpha- amylases in Vigna and Phaseolus. Abst. XV EUCARPIA Congress (20-25 Sept.1998, Viterbo, Italy) "Genetics and breeding for crop quality and resistance". P.39.

Konarev Al.V., N.Tomooka, and Duncan A.Vaughan (1998) Polymorphism of proteinase inhibitors in representatives of subgenus Ceratotropis of genus Vigna Savi. International Conference on Introduction and Distant Hybridization of Plants. (Main Botanical Garden RAN, Moscow, Dec. 15-17, 1998) (In Russian).

Al.V. Konarev and Fasulati S.R.(1996) Proteinases of colorado potato beetle and their plant proteinaceous inhibitors. Abstr. XX Int. Congr.of Entomology, Firenze, Italy, Aug 25-31, 1996

Al.V. Konarev Al.V. (1995) Interaction of insect digestive enzymes with protein inhibitors from plants and host-parasite coevolution. Abstracts. Adaptation in plant breeding. XIV EUCARPIA Congress, Juvaskyla, Finland, July 31-August 4., 1995. P.48.

Al.V. Konarev., Fasulati S.R. (1995) Inhibitors of alpha-amylases and proteinases and resistance of cereals and potatoes to insects. XIII International Plant Protection Congress. Abstracts 2-5 July, 1995, The Hague, The Netherlands P. 169

Al.V.Konarev (1995) Proteinaceous hydrolase inhibitors in mechanisms of plant defense. Abstr. All-Russian Plant Protection Congress. St.Petersburg, December, 1995), 1995. 205-206.

Al.V.Konarev (1995) Insect Digestive Enzymes and Their Protein Inhibitors in Cereals. In Edited by C. Michael Smith, Head, and John Reese, Professor, Dept. of Entomology, Kansas State University, Manhattan, Kansas ANNUAL PLANT RESISTANCE TO INSECTS NEWSLETTER. VOL. 21. 1995.

Al.V.Konarev (1995) Digestive enzymes of phytophages and their proteinaceous inhibitors as factors of biochemical diversity of animals and plants. Abstr. Meet. FACTORS OF TAXONOMIC AND BIOCHOROLOGICAL DIVERSITY. St.Petersburg. Zoological Inst. RAN, Apr 12-14, 1995. P.39.

Konarev Al.V.(1994) Hydrolase inhibitors in plants: new approaches and prospects. Abstracts of Int. Biotechn. Gen. Engineering. Symp. Kiev, 1994.

Konarev Al.V.(1994) Replicas for the detection of the alpha-amylase and proteinase inhibitors and lectins after analytical fractionation of proteins in gels. Abstr. of International conference "Biotechnology St.Petersburg'1994. (St.Petersburg, Russia, September 21-23, 1994), p.86-87.

Al.V.Konarev (1994) Digestive alpha-amylases and proteinases of insects and their protein inhibitors in cereals and other plants. Abstracts of 5th European Congress of Entomology. University of York, UK. 29 August - 2 September 1994.

Al.V.Konarev (1991) Hydrolase inhibitors and problems of wheat and other cereals evolution. Materials of All-Union symp. "Systematics and evolution of cereals (USSR, 1991) (In Russian)

Al.V.Konarev., Yu.V.Fomicheva (1991) Hydrolase inhibitors and coevolution of cereals and harmful pests. Materials of IX All-Union Conference on Plant immunity to decease and pests (Minsk, USSR, 1991) (In Russian)

Al.V.Konarev (1988) Systems of exogenous hydrolase inhibitors in wheat. Proc. All-Union conference "Physiological and biochemical basis of immunity of plants to fungi deceases (Ufa, USSR, 1988) (In Russian).

Al.V.Konarev (1987) Polymorphism of alpha-amylase and proteinase inhibitors and possibilities of its use in variety identification of cereals. Proc. III International symposium ISTA (Leningrad, USSR, 1987)

Al.V.Konarev (1987) Genetic control and variability of alpha-amylase and proteinase inhibitors in wheat, aegilops and rye. Proc. V. Congress N.I.Vavilov VOGiS. V.IV. (Moscow, 1987) (In Russian)

Al.V.Konarev, N.A.Vilkova (1986) Hydrolase inhibitors and wheat immunity. Abstr. VIII All-Union conf. "Immunity of agricultural plants to deceases and pests. V.1. (Riga, USSR,1986) (In Russian)

Al.V. Konarev (1986) Evolutionary and genetic aspects of studying of polymorphism of proteinase and alpha-amylase inhibitors in wheat and related cereals. Proc. III All-Union Conf. Chemosystematics and evolutionary biochemistry of higher plants (Gagra, USSR, 1986) (In Russian)

Al.V.Konarev, N.A.Vilkova (1984) Ecological and genetical aspects of study of inhibitors of enzymes of insects and other organisms in cereals. Abstr. II All-Union Conf. Ecological genetics of plants and animals (Kishinev, USSR, 1984) Kishinev, Shtiintza (In Russian)

Al.V. Konarev (1982) Properties of alpha-amylase inhibitors in relation with problems of systematics and phylogeny of festucoid cereals. Abstr. II All-Union Conf. Chemosystematics and evolutionary biochemistry of higher plants. (Yalta, USSR, 1982) Moscow, GBS AN USSR (In Russian)

Al.V. Konarev (1982) Genome specificity and genetic control of alpha-amylase inhibitors from grain of wheat and it relatives. Abstr. IV Congr. N.I.Vavilov VOGiS (Kishinev, USSR, 1982) Kishinev, Shtiintza (In Russian)

Al.V. Konarev (1981) The nature of alpha-amylase inhibitors in relation with problem of immunity of wheat and other cereals. All-Union Conf. Problems and paths of increasing of plant resistance to deceases and extreme conditions of environment in relation with tasks of selection (Leningrad, USSR, 1981) Leningrad, VIR (In Russian)

Al.V.Konarev (1981) alpha-amylase inhibitors and their role in protection of cereal grain from pests. Abstr. VII All-Union Conf. Immunity of agricultural plants to deceases and pests (Novosibirsk, USSR, 1981) (In Russian)

Patents

BRADY, R.L., SHEWRY, P., LUCKETT, S., KONAREV, A. CLARKE A., NASH, D.
PEPTIDE INHIBITOR OF BROWMAN-BIRK TYPE [Bowman-Birk]
WO 00/31139 (13) A1 PCT/GB99/03945
25 November 1999 - 02 June 2000

USSR Author licences

Al.V. Konarev, Yu.V. Fomicheva., N.A. Vilkova (1991) Method of identification of hydrolase inhibitors. USSR Patent (Author license) (In Russian)

Al.V.Konarev (1990) Method of identification of lectins. USSR patent (In Russian)

Al.V.Konarev, A.V.Romanuk, V.G.Konarev (1989) Method of identification of proteinase inhibitors. USSR Patent (In Russian)

Al.V.Konarev, N.K.Gubareva, N.A.Vilkova (1989) Method of wheat variety identification. USSR Patent (In Russian)

Al.V.Konarev (1986) Method of identification of proteinase inhibitors. USSR Patent (In Russian)

Al.V.Konarev (1985) Method of identification of proteinase inhibitors. USSR (In Russian)

Al.V.Konarev (1985) Method of identification of alpha-amylase inhibitors. USSR Patent (In Russian)

Al.V.Konarev (1982) Method of identification of alpha-amylase inhibitors. USSR Patent (In Russian)

The Soviet journals Prikladnaya Biokhimiya i Microbiologia, Doklady VASKHNIL, Biokhimiya, Cytologia and Genetica were translated in English by Allerton Press, Plenum Publishing Corp. etc.

 

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